Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos
نویسندگان
چکیده
منابع مشابه
Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded...
متن کاملSomatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.]
Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish n...
متن کاملSoybean [Glycine max (L.) Merr].
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
متن کاملSomatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0...
متن کاملCallus Induction and Organogenesis in Soybean [Glycine max (L.) Merr.] cv. Pyramid from Mature Cotyledons and Embryos
Soybean is one of the most important legumes of the world. Soybean plants are affected by several biotic and abiotic factors as well as insect pests and diseases which lower the quality and production of the crop. In order to overcome these biotic and abiotic challenges, a systematic crop improvement plan has to be followed in order to enhance crop production which involves the use of new techn...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2006
ISSN: 1516-8913
DOI: 10.1590/s1516-89132006000100006